Pgem t easy ampicillin

. and cloned into pGEM-T easy vector system (Promega, USA). Randomly, 10 ampicillin positive clones were screened through restriction digestion and two clones.

plasmide pGem-T (pGem-T Easy Vector System, Promega) ou le plasmide pBluescript KS+ (Stratagene). La réamplification des fragments est.

Immunohistochemical and genetic approaches for

[Biologie Moléculaire] Problème de clonage dans le pGEM-T easy vector. Sinon pour l'ampicilline, si tu l'as fait en septembre,.T E C H N IC A L N O T E Development of single sequence repeat markers for the ant. puriÞed and ligated into a clon ing vector using pGEM -T Easy Vector II.Temperature adaptations in psychrophilic, mesophilic. mesophilic and thermophilic chloride-dependent. and the resulting PCR fragment was cloned in the pGEM-T Easy.pGEM(T)-easy and removal of the whole TBAg cassette. The synthetic TBAg cassette was then inserted into a plant expression system. The TBAg cassette was first.

Functional evolution of scorpion venom peptides with an

UNIVERSITE DE STRASBOURG THESE DE DOCTORAT Présentée à la FACULTE DES SCIENCES DE LA VIE. I.9.8.4 Cloning by pGEM®-T Easy Vector System I 84 I.9.8.Ampicillin baownbeuv uses ampicillin elimination ampicillin 250 mg iv ampicillin gegen durchfall pgem. Ampicillin marker scaft salmonellen ampicillin bi?t du?c.

Elodie HENRIET, Commissariat à l'Energie Atomique (CEA

J'ai relu le protocole pGEM-T easy et dans le troubleshooting,. L'ampicilline a été fait en mi-septembre,.Development of a Nuclear Transformation System for Oleaginous Green Alga Lobosphaera (Parietochloris) incisaand Genetic Complementation of a Mutant Strain.

DOCTEUR DE L’UNIVERSITE D’ORLEANS

Sensitive measure of prevalence and parasitaemia of haemosporidia from European blackbird (Turdus merula) populations: value of PCR-RFLP and quantitative PCR.

Calreticulin contributes to C1q-dependent. into pGEM T-easy vector and cloned into JM109 cells accord-ing to the manufacturer’s instructions (Promega, USA).pGEM-T and pGEM-T Easy Vector Systems Technical Manual #TM042.pdf: Download. Promega-pgemt-easy-manual.pdf - Use only the T4 DNA Ligase provided with the system,.

Centrifugation at 30,000 x g in plasmid DNA precipitation

bulk soil DNA were used for cloning into the plasmid vector pGEM-T (pGEM-T Easy Vector System kit; Promega) in order to generate a 16S rRNA clone library.Presence and diversity of anammox bacteria in cold hydrocarbon-rich seeps and. the pGEM T-easy vector. agar plates containing 100μM of ampicillin,.FAD2BnF1 and FAD2BnR1 were ligated to pGEM ®-T Easy cloning vector using a pGEM-T Easy Vector. and the cells plated on LB-agar plates containing ampicillin,.pGEM-T Easy vector, and the labeled RNA (antisense) probe was synthesized with a STRIP-EZ RNA kit (Ambion), using the T7 promoter for rpl11 and the.

thesis Dec. 2009 - ori-nuxeo.univ-lille1.fr

Expression in E. coli and characterization of the catalytic domain of Botrytis cinerea chitin synthase. Herv e Magellan, Thierry Drujon, Annie Thellend, Annie Pi.Le pGEM-T est un vecteur « high copy » composé de la séquence oriC, du gène amp responsable de la résistance à l’ampicilline,.PCR products were then cloned into the pGEM®-T Easy Vector (Promega) before transformation of XL-1 Blue. ampicillin medium. Then, the recombinant clones were.B.T.S. Année 1. Mathématiques;. ampicilline et tétracycline qui peuvent servir l'un et l'autre de marqueur de recombinaison ou de. PGEM-Luc PGEM-T pCMVTNT 4.

T4 Dna Ligase Promega.Pdf - thebookee.net

T E C H N IC A L N O T E - p0.storage.canalblog.com

transformation: ampicilline marqueur de recombinaison: lacZ. Quelques vecteurs utilisés en Biologie Moléculaire. 1. PGEM-T iv. PCMVTNT. Author: LSD Ghost.

On the identity and origin of the Mediterranean invasive

The presence of the seahorse Hippocampus patagonicus in

Diversité microbienne d’un écosystème complexe

0/Le système de sélection sur ampicilline: la présence du gène de résistance à l’ampicilline dans PGEM-T permet aux bactéries ayant intégrées la.

3.4) Vecteur de Clonage pGEM-T-Easy. Amp: ampicilline ANA: acide α-naphtylacétique BAP: benzylaminopurine BSA: albumine de serum bovin.A1360 pGEM®-T Easy Vector System I 20 réaction NA.51 PCR 1 202,00 15,00 171,70. A3610 pGEM®-T Vector System II 20 réaction NA.51 PCR 1 265,00 15,00 225,25.purified and ligated into a cloning vector using pGEM-T Easy Vector II (Promega). Glenn TC, Cary T, Dust M, Hauswaldt S, Prince K, Clifton R, Shute I.ACCELERATED PUBLICATION Transcriptomic analysis in the leech Theromyzon tessulatum: involvement of cystatin B in. have cloned the full-length cDNA and named the T.On the identity and origin of the Mediterranean invasive Caulerpa racemosa (Caulerpales, Chlorophyta). PCR products were then cloned into pGem-T Easy.

Progressive osseous heteroplasia: a model for the

THESE pour le DIPLÔME D’ETAT DE DOCTEUR EN PHARMACIE